NSP.scaling_factor

missionbio.mosaic.algorithms.nsp.NSP.scaling_factor

NSP.scaling_factor(reads: ndarray) → Tuple[float, list]

Identifies the appropriate scaling factor for oversequenced runs.

Parameters:

readsnp.ndarray

The protein read counts to be normalized. The shape should be (#cells, #antibodies)

Returns:

float

The factor to scale down the reads by.

list

The indices of the antibodies in which the peak amplification was observed at the identified scaling factor.